FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_zk-hu-0163.351000000a1237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_zk-hu-0163.351000000a1237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128095
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA4700.36691518013974006No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC3440.26855068503844803No Hit
GTATATAACACTTGTTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC2710.21156173152738203No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC2090.16316015457277802No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2040.15925680159256803No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC1950.15223076622819No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC1860.14520473086381203No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG1650.12881064834693No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC1640.128029977750888No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC1600.12490729536672No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG1540.120223271790468No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA1530.11944260119442603No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT1490.11631991881025801No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC1460.11397790702213201No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC1400.10929388344588001No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA1350.10539053046567003No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA1300.10148717748546No Hit
CATTAAGATTGGTGCTTACAGCACTCCAGGTGTAGACCCTTCTTCACCCA1290.10070650688941803No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC1290.10070650688941803No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACC100.0070836945145.04
GGCTTCT606.77187E-660.416677
GTATCAA805.8331534E-754.3751
TCTTCCG553.208396E-452.7272725
AGGTTTT1053.7979844E-641.428579
CTTCCGT750.00131379838.6666641
TGTCGGA1502.5693225E-838.6666649
AGTGTCG1553.4300683E-837.4193577
TTCCGTG800.00203000236.252
ACTCATA800.00203000236.251
AACAGGT1001.3846857E-436.256
ACAGGTT1001.3846857E-436.257
TACTAGT1655.947004E-835.1515163
AACAGCC1251.2565119E-534.8145
CGGCTCG850.002733108434.117645145
ATACTAG1707.732342E-834.1176452
GATACTA1759.975338E-833.1428571
TCTGCAC1102.4243648E-432.9545443
GTATGTG900.00361651932.222227
TATGTGT900.00361651932.222228
Produced by FastQC (version 0.11.2)