FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_rt-pcr_neg_ctrl.351000000a1053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_rt-pcr_neg_ctrl.351000000a1053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTAATTTCGATCATCAAAGAAGCAGCTGACAACATTCGATTTTTCCAT50.41841004184100417No Hit
CCTTCAAGCAGATCGTTTTGAACATTCATCTTCAACCGATTCCAGCCCAT30.2510460251046025No Hit
CTGCAAGTTGACCAAGGGGGATGGTTTCCTTATCCCCCATCTCGGCCTGC30.2510460251046025No Hit
GTCTCTGCAAGTTGACCAAGGGGGATGGTTTCCTTATCCCCCATCTCGGC30.2510460251046025No Hit
GTATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAA30.2510460251046025No Hit
ATTTTATTGGTTCCAGAAAGGAAGAGCAGGATGGATAATTTGCTTACAAT30.2510460251046025No Hit
GCTCTGTGCCTTCGCCGATACCAAGCCTGTCGCACTACGGTGCAGATGGT20.16736401673640167No Hit
AGCAGGCACTTGCGGCAGATTTAGTTTCAAGCAGAGAAGGAAAAGAGGCA20.16736401673640167No Hit
CTTCAATCTCATCCAGCAAAATCAAAAATGGATGTTCATTTTTTGCAGCC20.16736401673640167No Hit
GTGCTGAAGCTGTACCGGCAAACCCGAATCTGCCGAAACCAATGCCGCCG20.16736401673640167No Hit
CAGAAGCACAGCGCGAAGCTGTAAAAGAAATCATTCAGAATGTCAGACAA20.16736401673640167No Hit
TCTTTATTCGATCCTCTCTATAACGCCTTGTAATTTACTACGCCATGAGC20.16736401673640167No Hit
GTTTCAAGCAGAGAAGGAAAAGAGGCAGCCGGAGAACTGGCCAAGCTTTG20.16736401673640167No Hit
GTCTGACAATGTCACGTTCTTCCTCGGTCAGCTCAGCCTCAATACCTGTG20.16736401673640167No Hit
TTCCTGCAGAGTTCAGAAATGCCTTTTCACTATAAGAAATAATGCTTGAT20.16736401673640167No Hit
GACCAAGGGGGATGGTTTCCTTATCCCCCATCTCGGCCTGCACGGTTGAT20.16736401673640167No Hit
AGTAGAAACAACAGCCAGATGAACAGTCTCTGCAAGTTGACCAAGGGGGA20.16736401673640167No Hit
GGAAAAATAGCAGCGTTCGCTCGTTTAGAAGGATTAGAAGCACATGCCCG20.16736401673640167No Hit
GTATCTGCCTAGAAAAATGGCTCCAGCATGCCGAATGCTGCTAAGTAAAT20.16736401673640167No Hit
TTACACAGGAATACTGTTTGAAGTCTACTCTGGAAATGTAGGTTTTCCGC20.16736401673640167No Hit
ATTGAACTGATTCGTCAGGGTATCGAGCATGCCGAACAAAATTCGCAATT20.16736401673640167No Hit
GTTCAATACTCCGGCAGCAGCTACAGGCTTCGGCATCAGGCTTGACCGGC20.16736401673640167No Hit
GCCATGAGCAGCGTACCTTACAGAGCGGTTTTGTTGTAATTAGTATTATT20.16736401673640167No Hit
ACCTTACAGAGCGGTTTTGTTGTAATTAGTATTATTTTGTAGGTAATTGC20.16736401673640167No Hit
TGAATCAGGAGGCGAAATGAGGACAATCCGCTTTACACCTGCTGTCTGTG20.16736401673640167No Hit
GTATGCGGCTGTACCCCCAGGAACATAAACCCCGACTGCATCAATCGGTG20.16736401673640167No Hit
ATCCAAGATTATGAGGATCTTCAATCTCATCCAGCAAAATCAAAAATGGA20.16736401673640167No Hit
GCACCAGCTTGCCGCTGGCGGCGTCGTAGTCCACCAGGCGGTTCATCAGC20.16736401673640167No Hit
GTATAGCAGTGGTCGGCATGATCGCATGATTGAACTGATTCGTCAGGGTA20.16736401673640167No Hit
ACAGCCAGATGAACAGTCTCTGCAAGTTGACCAAGGGGGATGGTTTCCTT20.16736401673640167No Hit
ACGGTTACGTGCCCGGTTGGCGGCGTTGGTATTCGGCGCCAGGGGGTAGC20.16736401673640167No Hit
TGCAAGAGCTGCAACGGCTTGAGCTCCCCCCGCTTTGTAGATTTCTTTTA20.16736401673640167No Hit
CTTCTGTGATAATCGGTTGAGTCAGATGTTTTTGCGCGGTATCTGCAATC20.16736401673640167No Hit
CTACCTGGCTGTTGCAGGGCTTCCGGAGGCAAACGGATCTGATGTGGTTG20.16736401673640167No Hit
CAGATGAACAGTCTCTGCAAGTTGACCAAGGGGGATGGTTTCCTTATCCC20.16736401673640167No Hit
TAAGCGTAAGGTGCCGCTTGCGCCACCACTCCCTGGTGTTGAACGCCCGG20.16736401673640167No Hit
CAATAAATGGCTGTTGCTGCTGAGTGCCAAGTCGTTTTCCCACCATCATG20.16736401673640167No Hit
GTGTCAGCAAGGCGCTAGAACGGCTGAACATTGGCTATTTTATATCAGAA20.16736401673640167No Hit
GTTTTGGATACGGTCACTGTAACTGCAGCTGAGCGACGTTTCGGTACAAT20.16736401673640167No Hit
GTGAGAATACGGCTCTGTGCCTTCGCCGATACCAAGCCTGTCGCACTACG20.16736401673640167No Hit
TGCTAGATTTATTTACGAAGCACGGTCAATTTGATTTAACAGTCGCAGCA20.16736401673640167No Hit
ATATTGGGGATGCGGTATATGAGGTAGCCGTACGACAGTATCTGTTATCT20.16736401673640167No Hit
GGTATTGAGGCTGAGCTGACCGAGGAAGAACGTGACATTGTCAGACAAGG20.16736401673640167No Hit
TCCCAATGCTTTAAAAACCGCTTCAATCATATGATGGGTATTGCGTCCGT20.16736401673640167No Hit
CTCTATAACGCCTTGTAATTTACTACGCCATGAGCAGCGTACCTTACAGA20.16736401673640167No Hit
AGTAGAAACAACAGCCGGAACCTCAACACCATAATCTGCACTGGCCAAAA20.16736401673640167No Hit
GCTCGATACCCTGACGAATCAGTTCAATCATGCGATCATGCCGACCACTG20.16736401673640167No Hit
ATTTGATTCTGTTCGTTTAGCGGATCTTAGAGTATCTGCTTCTGAAATTG20.16736401673640167No Hit
AATAAAGATGGAAGAAGAATGGATCGCCGGTAAGCACTCCGTGACGGAGG20.16736401673640167No Hit
CTTCCATTGTATCTGCCACATAAATGCATCCGTACTGTTCAATAGAAGGA20.16736401673640167No Hit
GTAGAAACACGAGCAACGGGCACGTACTCTACCGCACCTGCAGATGTTTT20.16736401673640167No Hit
AGCTGCAACGGCTTGAGCTCCCCCCGCTTTGTAGATTTCTTTTACACCAA20.16736401673640167No Hit
GAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTATTTAT20.16736401673640167No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)