FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_prvabc59b.351000000a12a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_prvabc59b.351000000a12a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158655
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5270.3321672812076518No Hit
GTATCATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC4640.29245847908984907No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4230.2666162427909615No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC3150.1985440105890139No Hit
GTCATAATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT3090.1947622199111279No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC3050.1922410261258706No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2930.18467744477009862No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC2460.15505341779332513No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC2240.1411868519744099No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC2130.13425356906495226No Hit
GTATTATGACCAAGTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGC2120.13362327061863793No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2100.13236267372600927No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2090.13173237527969495No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAA1870.11786580946077968No Hit
GAATAAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCA1820.11471431722920804No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG1790.11282342189026505No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC1770.11156282499763638No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC1760.11093252655132206No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG1660.10462954208817876No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC1620.10210834830292144No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT100.0070852796145.02
CACGATA100.0070852796145.03
ACGTCTG100.0070852796145.0145
CGATACT100.0070852796145.05
GTATTAT502.2562745E-887.01
AACCCTT702.7921487E-972.55
CTCAACC754.820322E-967.6666642
GTCATAA651.39049E-766.923071
TTATGAC702.3219218E-762.1428574
ATTATGA702.3219218E-762.1428573
TAATACT753.7409336E-758.05
TCTTCCG753.7409336E-758.05
ATAATAC753.7409336E-758.04
TCATAAT753.7409336E-758.02
AATACTG753.7409336E-758.06
CTGTAAC400.006041542654.3751
TGTAACT400.006041542654.3752
GCTACGA1004.6697096E-850.75145
CTTCCGT901.3164827E-648.3333326
TTCCGTG951.910612E-645.7894747
Produced by FastQC (version 0.11.2)