FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_pa-259459.351000000a1200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_pa-259459.351000000a1200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190320
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5510.2895124001681379No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4060.21332492643968054No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2930.1539512400168138No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC2610.13713745271122318No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2490.13083228247162673No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC2280.11979823455233292No Hit
CTATTAGGGTCAGGGGTGTTAATTGTGAGTAGCAACCTATCATTAGCAGC2220.11664564943253469No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC1990.10456073980664145No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCACGCGATGGCAG1980.10403530895334175No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA1910.1003572929802438No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCT100.0070863836145.09
GCGGGGT100.0070863836145.08
TTAACCC100.0070863836145.04
GTAAGTA100.0070863836145.01
TAGTACT100.0070863836145.04
CGAGTTT203.861196E-4108.754
TCGAGTT203.861196E-4108.753
TTCGAGT203.861196E-4108.752
GTTCGAG259.3749905E-487.01
TCTTCCG502.2990625E-672.55
TTCCGTG651.0902644E-555.769237
GACGGTG805.848342E-754.3757
GGACGGT805.848342E-754.3756
TATAGGA1600.049.843752
CTTCCGT901.3178815E-648.3333366
ATATAGG1650.048.3333361
ATATAAA450.00962723748.3333362
CGATAGA1056.867049E-848.333332145
TGTAGGT951.91264E-645.7894742
GGTTATT1151.4045145E-744.1304366
Produced by FastQC (version 0.11.2)