FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_p6-740.351000000a11f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_p6-740.351000000a11f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142475
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGATGAGGGGGTAGAACCAGATGACGTCGATTGCTGGTGCAACAC2770.19442007369713984No Hit
CTATATGTACTTAGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA2550.17897876820494824No Hit
GTATAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAAGTTGGAGTGG2170.15230742235479908No Hit
CTTAATACATTCACCAACCTGGTGGTGCAGCTCATTCGGAACATGGAGGC2010.14107738199684156No Hit
TACATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCCCCCT1900.13335672925074574No Hit
CATATACTCATCTCCAGGTTTGTTGGGATTCCTGCCTATGCGCCCCCTCC1720.1207229338480435No Hit
CTCTATACACTCCATCTGTGGTCTCCCCCTTTTTTACTTCCTTGGGAGCA1710.12002105632567116No Hit
ACTCTATATCAACAGTTGGCTTGTCCTGTGCCATTACGGTAACACAACCT1550.10879101596771364No Hit
ACATGTACTTGATCCCAGGTCTACAGGCAGCAGCGGCGCGCGCTGCCCAG1450.10177224074399016No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCAAT100.007084525145.01
TCTTCCG607.982271E-872.55
CTTCCGT651.3894714E-766.923076
AAAGCCT350.003560230162.1428574
CCTAGAA752.5394887E-548.3333322
GTGGTTA450.00962230748.3333328
TCATATA700.001053589741.428572
AGATGTT1401.39989424E-841.42857145
TGAGTGT1105.2325504E-639.5454526
CCTTATG750.001479115338.6666641
AGAAAAT951.02445796E-438.157896
ACCTACA951.02445796E-438.157892
AAATGAC951.02445796E-438.157899
ATTGAGT1157.099714E-637.8260884
GTCATAT800.002030767736.251
TATTGAG1209.506402E-636.253
CAGATAG1001.3854683E-436.259
ACAGATA1051.8457675E-434.5238088
CGGCTCG1102.4257289E-432.954544145
AAAATGA1102.4257289E-432.9545448
Produced by FastQC (version 0.11.2)