FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_ny238_plasmid.351000000a12e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_ny238_plasmid.351000000a12e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167151
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG4310.257850685906755No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG4300.2572524244545351No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC3730.22315152167800373No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA3510.20998976972916705No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG3210.19204192616257157No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT2750.1645218993604585No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA2460.1471723172460829No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT2250.13460882674946606No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT2200.1316175194883668No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG1980.11845576753953012No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC1930.11546446027843088No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT1870.11187489156511178No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG1870.11187489156511178No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1800.10768706139957283No Hit
CTTCTAATAGATGGCACAGCATCATTGAGCCCTGGGATGATGATGGGCAT1780.10649053849513314No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1770.10589227704291329No Hit
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATC1760.10529401559069346No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG1750.1046957541384736No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1750.1046957541384736No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT1730.10349923123403389No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG1720.10290096978181405No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT1680.10050792397293465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTAGT100.0070856186144.999981
TAATGTA202.1253636E-6144.999985
GTATTAA256.4490087E-6116.01
GTATACC301.5955458E-596.6666649
GATGATA259.372977E-487.0145
TGCTCTA406.646979E-572.499999
TACTGAT1350.064.444447
TCCTAAT350.003561310762.1428571
ATACATC502.005382E-458.03
TAGGACC400.00604210954.3749969
CATATAC2150.047.20933
TATACTG2300.044.1304365
ACTATAA1351.0171789E-842.9629633
ATACTGA2200.042.840916
TCCCGTG1201.9602885E-742.2916687
ATATACT4400.039.5454564
GGACTAT2057.2759576E-1238.902441
CCTAGAA750.001479850338.6666682
GACTATA1502.577326E-838.6666682
ACTGATG2600.036.258
Produced by FastQC (version 0.11.2)