FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_mr766a.351000000a1294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_mr766a.351000000a1294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176396
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG4120.23356538697022608No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA3740.21202294836617613No Hit
GAATGGTTTTGGCGATACTAGCCTTTTTGAGATTTACAGCAATCAAGCCA2980.16893807115807613No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGAGCTTGGTTTCC2710.15363160162361958No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA2470.14002585092632486No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA2430.13775822581010908No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA2240.1269870065080841No Hit
CCCACAAGGTGGAGGTCGCTGCTGTGATCAGAGCTCCAGCCTCCCCCCAT1900.1077121930202499No Hit
CATTAATAGGGTCTACTACCCTCACAGCTGTCAATCCCAGGGCCATGACA1900.1077121930202499No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC1860.10544456790403411No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT1850.10487766162498016No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG1790.10147622395065647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATG100.007085945145.000024
TTCTTAT100.007085945145.000023
TACTCTG100.007085945145.000025
TTCTCAT100.007085945145.000022
CCTTATG404.793037E-9108.750011
TCTTCCG607.2759576E-1296.6666645
TTATGGG451.0864824E-896.6666643
GGCTTCT651.4551915E-1189.230771
CTTCCGT755.4569682E-1177.333336
AGTCTGA406.64799E-572.500011
AAGCGAG451.191144E-464.444447
TCCGTGC902.8012437E-1064.444448
TGAAGCG502.0056868E-458.05
GAAGCGA553.2117253E-452.7272726
CTGAAGC553.2117253E-452.7272724
GTATAGA1351.891749E-1048.3333361
TCATGCG752.5419722E-548.333332145
TAGATAA1402.6921043E-1046.6071434
TATGCAC1251.2592007E-534.85
TTGAGTG1251.2592007E-534.85
Produced by FastQC (version 0.11.2)