FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_mex-i-44.351000000a1251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_mex-i-44.351000000a1251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207031
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5670.27387202882660083No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4420.2134945974274384No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC3500.16905680791765484No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC3290.15891339944259555No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT3230.15601528273543575No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC3220.15553226328424247No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC2790.13476242688293058No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2730.13186431017577077No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2730.13186431017577077No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC2700.13041525182219088No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC2660.12848317401741768No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC2540.12268694060309808No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2480.11978882389593828No Hit
ATAATATACAGATTGTAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA2300.1110944737744589No Hit
ATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAG2210.1067472987137192No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC2200.1062642792625259No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA2130.10288314310417282No Hit
GATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAG2080.1004680458482063No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTATC100.00708683145.0145
AACTAAT100.00708683145.0145
GGCTTCT650.0100.384621
TTTTCCG259.3761605E-487.09
GAAGCGA451.2294222E-680.555566
AGTTTGA451.2294222E-680.555561
GCGAAAG502.2997756E-672.59
AAGCGAA502.2997756E-672.57
GCTTTTT300.001933566572.56
CTTCCGT955.456968E-1268.684216
TCTTCCG955.456968E-1268.684215
GTTTGAA554.0502455E-665.909092
CGCAGGC350.003562510262.1428572
TGAAGCG606.786606E-660.4166685
TTCCGTG1200.060.4166687
CCCTGTA1505.329639E-1043.52
GGTTCTG855.3267686E-542.647067
CCTGTAC1557.348717E-1042.096773
TATATTA1601.0022632E-940.781252
TATTATG1601.0022632E-940.781254
Produced by FastQC (version 0.11.2)