FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_mex-2-81.351000000a1244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_mex-2-81.351000000a1244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180781
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA6110.3379779954751882No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4810.26606778367195666No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC3890.21517747993428515No Hit
GCCCAGGTGGTGCGCGGTCTGTGCCCTATGAGAGATCCACACCACAAGTC3740.20688014780314304No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC3730.20632699232773355No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT2630.1454798900326915No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2450.13552309147532096No Hit
CAGCATGGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTG2410.13331046957368306No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2380.13165100314745465No Hit
CATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAA2210.12224736006549361No Hit
GATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGC2180.12058789363926518No Hit
GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGA2170.12003473816385572No Hit
GTCTGATATTGATGCCTCATAGCAGTAGGATCTTACCTCCGCCATGTTGC2130.11782211626221782No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC2080.11505633888517046No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC2010.11118425055730413No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC2000.11063109508189467No Hit
GTACATATAGTATGCACTCCCACGTCTAGTGACCTCCGCTGCCATAGCTG1910.1056526958032094No Hit
GTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTATTTTG1890.10454638485239046No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA1880.10399322937698098No Hit
GTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGA1860.10288691842616204No Hit
CATTAAGATTGGTGCTTACAGCACTCCAGGTGTAGACCCTTCTTCACCCA1810.10012114104911468No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT259.37422E-487.04
GTTCGAG259.37422E-487.01
TCGAGTT259.37422E-487.03
TTCGAGT259.37422E-487.02
GGCTTCT953.1201125E-853.4210557
ATATAGG1405.456968E-1251.7857131
GATAGAA1152.4647306E-950.434781
GTTTGAA450.00962646148.3333362
GCGAAAG450.00962646148.3333369
TATAGGA1509.094947E-1248.3333322
TCTTCCG902.1776423E-540.277789
GTATCAA1105.240383E-639.5454521
GTATGAG750.001480169838.6666642
GGCATAG750.001480169838.6666641
CTTAGAT750.001480169838.6666643
ATATACT1752.406523E-937.2857134
ATATGTA3900.037.179493
TATGTAC3900.037.179494
CTATATG4700.037.021281
CAGTTAG800.002032211336.2500049
Produced by FastQC (version 0.11.2)