FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_ibh-30656.351000000a122a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_ibh-30656.351000000a122a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229417
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTCGTTA3380.147329971187837No Hit
TATTTACACAGTGACAAGAAATGCCGGTCTGGTTAAGAGACGTGGAGGTG3160.13774044643596595No Hit
ATTCATTAGAGTGTGACCCAGCCGTCATAGGAACAGCTGTCAAGGGAAAG3060.13338157154875183No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA2400.1046129972931387No Hit
TCATAGTACTGGTGTATGGGCACAGCACCTTTATGCAGAAGGCCCCTGGT2370.10330533482697446No Hit
CTAATGAATAGTCCTCTCTGACCTTCAGCCAAACACTGGTGTGGAAGACC2360.10286944733825305No Hit
GTGTAAATAAGGGAAGCCCCTGCCAAATAACTTCCTCTGAAGATATTGCA2330.10156178487208882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGCC100.007087326145.0145
GGCTTTT702.7994247E-972.51
GTTCGAG502.3005705E-672.51
CGAGTTT300.001933835172.499994
TCGAGTT300.001933835172.499993
TTCGAGT300.001933835172.499992
GCTTTTT808.049028E-963.43752
TTTTCCG851.299486E-859.7058835
TCCGTGC953.1252057E-853.4210558
CTAACAC1451.9166691E-840.03
CTCTAAC1451.9166691E-840.01
GATACCA1251.2608511E-534.81
CTCTTTA1102.4303305E-432.9545481
TATTTAC2008.887582E-932.6251
TACCATC1352.1350586E-532.222223
ATTTACA2051.1305019E-831.8292682
CATCCCC1153.1545653E-431.52174145
TTTCCGT1652.3758603E-630.7575766
CTGCTTA950.004719785430.5263189
TCCTGAC1453.4788034E-530.08
Produced by FastQC (version 0.11.2)