FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_hrv16.351000000a12ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_hrv16.351000000a12ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76345
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTA259.3533116E-487.03
GGTACCG300.001928872972.55
GTAATAG350.003553893462.1428571
GTGTTCC400.00602957254.3759
ACTACTG400.00602957254.3755
GACCATT553.2001626E-452.7272727
TATACTG858.815714E-751.1764685
CTATACT604.916225E-448.3333324
TCTCAGG450.0096053148.3333321
GGACCAT450.0096053148.3333326
GGATTGT450.0096053148.3333326
ATACTGA750.001474805438.6666646
TGGCGTG950.004700267730.5263185
GTTTTAT1450.001216087424.9999981
AAAAAAA26300.05.4030423135-139