FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_hrv14.351000000a12bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_hrv14.351000000a12bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224884
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA450.00962949648.3333324
TACCGAG450.00962949648.3333327
TGGTCTA1354.9485425E-737.5925947
GTCTACA1301.6491062E-533.461549
TTCTAGA900.003622298832.222221
TGTATAC1153.154368E-431.5217363
TAGATCT1453.4784993E-530.04
GTATATA2202.2477252E-829.659091
ACCGCTC1753.7575228E-629.08
GTATACA1753.7575228E-629.01
AGGGCTA1050.00769595827.6190479
ATGGTCT1606.805008E-527.18756
CCGCTCG1907.123208E-626.7105249
AGACCGT1100.00965595726.3636366
AAGTATG1409.970447E-425.8928572
CTTACTG1701.0278139E-425.5882363
CTGATAC2001.0606871E-525.3753
ATAATGG2901.1801603E-825.03
ATATGAG1450.001223487925.05
CTAGATC1851.82453E-423.5135123
Produced by FastQC (version 0.11.2)