FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_hb-2016-1840.351000000a126d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_hb-2016-1840.351000000a126d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179868
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAA5410.3007761247136789No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4490.24962750461449507No Hit
GTATATAACACTTATTCATCCCCAATGTGGTTGGAAAAGATATGGCCTCC3550.1973669579914159No Hit
ATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGT3260.18124402339493406No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2540.14121466853470324No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATAGGCTTGGTTTCC2530.1406587052727556No Hit
ATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACC2330.12953944003380255No Hit
ATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACAC2260.12564769720016902No Hit
CCCCTGTACCGCATCTCGTCTCCTTCTTTGAGAAGTCCACCGAGCACCCC2190.12175595436653545No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2110.11730824827095425No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTAC1990.11063668912758245No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA1860.103409166722263No Hit
ATAATATACAGATTATAGTTGGGGACTCTGATTGGCTGTAGTAGACGTGA1840.10229724019836768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT351.893568E-9124.285714
GTTCGAG351.893568E-9124.285711
TCGAGTT351.893568E-9124.285713
TTCGAGT351.893568E-9124.285712
AGTTTGA554.373942E-879.0909046
GGCTTCT554.0480863E-665.909091
GTTTGAA753.743753E-758.0000047
TCTTCCG651.0900221E-555.769235
CTTCCGT701.690381E-551.7857136
TTCCGTG701.690381E-551.7857137
GCCTGGC450.00962638648.3333323
AAGCGAA500.001393246543.57
GCACGTC1354.941194E-737.5925946
CACGTCA1354.941194E-737.5925947
GAAGCGA600.00470154936.256
GGCACGT1406.569553E-736.255
ACGTCAC1458.645784E-735.08
TGAAGCG650.00799020633.4615365
GGTGTCG900.003620385232.222225
GTGTGGT900.003620385232.222221
Produced by FastQC (version 0.11.2)