FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_fss-13025.351000000a121d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_fss-13025.351000000a121d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184206
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAA4540.24646319881002793No Hit
GTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGC4230.2296342138692551No Hit
ATATAGGACATGGGCTTACCATGGAAGCTACGAGGCCCCCACACAAGGGT3130.16991846085360954No Hit
CCCCTGTACCGCATCTCGTTTCCTTCTTTGAGAAGTCCACCGAGCACCCC3030.1644897560340054No Hit
GTATATAACACTTATTCATCCCCATTGTGGTTGGAAAAGATATGGCCTCC3010.16340401507008456No Hit
GTATATGACTTTTTGGCTCGTTGAGCTTCCCAAAAGCCAAGCGATGGCAG2780.15091799398499506No Hit
GTACAGGGGTGTTCGTCTATAACGACGTTGAAGCTTGGAGGGACAGGTAC2640.14331780723754925No Hit
GTCCTATATGGGTGGTTCTCGTCAAAGAACCACGTTTCCGCGTGCTCACT2570.13951771386382636No Hit
ATATGTACTTGGACAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACC2430.13191752711638058No Hit
GTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAA2230.12106011747717228No Hit
GGCTTCTTCCGTGCCATGGCGTTCTCGGCCTGACTATGGGCTTGGTTTCC2060.11183131928384525No Hit
ATGCTGGATGAGGGGGTAGAACCAGATGACGTCGATTGTTGGTGCAACAC2060.11183131928384525No Hit
CTTAATACATTTACCAACCTAGTGGTGCAGCTCATTCGGAATATGGAGGC1980.10748835542816196No Hit
GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAG1880.1020596506085578No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGAT100.0070862006145.03
GCGTCAG100.0070862006145.01
CGTCAGA100.0070862006145.02
AGATGTG100.0070862006145.06
GGCTTCT451.0866643E-896.6666641
TCTTCCG554.3744876E-879.0909045
CTTCCGT502.2987679E-672.56
TTCCGTG502.2987679E-672.57
TAGGACA1252.7028364E-740.64
TATATGT3400.034.117652
GTATCAA850.002736209234.117651
ATATGTA3850.030.1298713
TATGTAC3850.030.1298714
CCGTACA1453.4751174E-530.02
CGTACAC1453.4751174E-530.03
TCCGTAC1504.3794935E-529.0000021
TTATGAT1000.00606177529.03
ACTCATA1255.137733E-429.01
ACTTGGT1753.7526697E-628.9999988
ACTTGGA4100.028.2926839
Produced by FastQC (version 0.11.2)