FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_extraction_neg_ctrl.351000000a116e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_extraction_neg_ctrl.351000000a116e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4362
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATGGCTCAACACTGAAATCTTGAGGATGAAAAACAGAATTTGC100.22925263640531865No Hit
ATATATGTGTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTACTTTC100.22925263640531865No Hit
GTAGTAACAAGAGCCGGCGCAGGCGTCGCTGCACCTGCCGCTGTGTTCGC100.22925263640531865No Hit
ACCGTAAACCTTGCAAACATAAGGATTATCAAGACAATTTCTTCTGACTC90.20632737276478677No Hit
CTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAGAGACACTA90.20632737276478677No Hit
GGCTATAACAATACTGGCCTATTTCATCAAGTTTTATTTTGTCCTGCTTT80.18340210912425492No Hit
GTAGAAACACGAGCACGAACACGAGCACGAACACGAATGCGAATGCGAAT80.18340210912425492No Hit
TAACTGGATGATGTCCTCTGGTCACAGTTTTACTTTCTTTTTTTTTTTTT70.16047684548372307No Hit
CTGCAAACTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCC70.16047684548372307No Hit
TCTTTGATCTGTTGCAGATGGTAGTGTTTGTTAACTCTGTCTGGTTTCCT70.16047684548372307No Hit
ACCAATGGGTGCTTAAAAATTTAATAACACTGAAGGCTCAGTTTCTTCTG70.16047684548372307No Hit
GCATAAGACAATCAATTTTGGGGGGAAAAAATCCCCAGTGTTCCCATAGT70.16047684548372307No Hit
GTCTTATGCAGGAATTTGGAGGTGACAGTAATAGCAGGATTACCGTAAAC70.16047684548372307No Hit
ATATTATAGATGTTAACTTTCCAGCAGGACTTTATTTTGAAAGGCACCTT70.16047684548372307No Hit
GTATAGGTATGGAGGGTATAGAGGGTGGCAGGTACCCAGTAGTAACTGTA60.1375515818431912No Hit
ATTGGGGACTTGGTGATTCACTATTTTGCGGGAAGAGGGAGGAGATGGGG60.1375515818431912No Hit
GAATAATGGCAATGGCAGTTGGACATGATTCTGTTTTTTTCTTGTTGGGT60.1375515818431912No Hit
GAAATAGACTTTGCCAGCATAGGGGATATTGTGTCTCCTGTAGGATCCAG60.1375515818431912No Hit
GGATATGAGAGTCCTGGACGACAGAATCCCGTGGAGTCTTGATCTCTCAT60.1375515818431912No Hit
ATTCCTACCTGTGACTAATTTGGTGTCTGTAGGGTGGAGTCTGAATTCCA60.1375515818431912No Hit
ACATAGAAAGTGAAACTGTGGGCCGGGCACGGTAGCTCATGCCTGTAATC60.1375515818431912No Hit
ATTCAGGGCAGTCCTCTGTGTCAATGCTGGATGCAAATGCACAGTACGAA60.1375515818431912No Hit
ACCCTACAGACACCAAATTAGTCACAGGTAGGAATATTATAGATGTTAAC60.1375515818431912No Hit
CTTTAAGACTTTCATTATCTTTGCCTGTTATTTTAGCTGCCTCTTAATTG60.1375515818431912No Hit
CTGTAGGGTGGAGTCTGAATTCCAAGAATTGAGTTCCCCATCTGTTAAGC60.1375515818431912No Hit
GTCCTATATATGTGTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTA50.11462631820265932No Hit
ATCACCAAGTCCCCAATGAAACCAGGAAACCAGACAGAGTTAACAAACAC50.11462631820265932No Hit
TTTTATAACTTCGGTAATGAAACACTAGCATAATTATATTTCTATTTAAA50.11462631820265932No Hit
AGTTAATACATTGGCTGTGGTGATGACTATGGGAACACTGGGGATTTTTT50.11462631820265932No Hit
GTCTATGTTTCTTACCATTTCTTTTCCCTGATAGATTCGACCATTTTCTT50.11462631820265932No Hit
GCACTAGCCCTGAATTAAAATTCTCCACTAGAGAGTCAGAAGAAATTGTC50.11462631820265932No Hit
GACTATGGGAACACTGGGGATTTTTTCCCCCCAAAATTGATTGTCTTATG50.11462631820265932No Hit
ATCCAGGACAGGGTCCCTCCCCCTTGAGGTACCTGCATTTATTTCCCCAT50.11462631820265932No Hit
CTGTTAAGCCAAGAAGTAATTTGAATCTTAATTCAGGGCAGTCCTCTGTG50.11462631820265932No Hit
TTCCCAACTCAGATCCCAGAACAGAGTCAGCCAGACTTGTACCTACCAGA50.11462631820265932No Hit
CATAGAAAGTGAAACTGTGGGCCGGGCACGGTAGCTCATGCCTGTAATCC50.11462631820265932No Hit
GCTTTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAG50.11462631820265932No Hit
GTTCTATTTTCACTGCACGACATCAATATATTCTCAAAAGAAATTAAGAG50.11462631820265932No Hit
TTACAGAGCAAGTCAAAACCAACTGGGACCCAACAAGAAAAAAACAGAAT50.11462631820265932No Hit
CTCCTCCCTCTTCCCGCAAAATAGTGAATCACCAAGTCCCCAATGAAACC50.11462631820265932No Hit
GTATTAACTAGTTTACAGGAAATGAAGCACTTCGTACTGTGCATTTGCAT50.11462631820265932No Hit
GTATGATAACATAAGTTTCACAAAACTTGAGACATGTCATCTTGGTTATA50.11462631820265932No Hit
CCCATCCCCTTTCCCAACTCAGATCCCAGAACAGAGTCAGCCAGACTTGT50.11462631820265932No Hit
TTGCTAGATTAAGTGAAAAATCTACTCATGGAATGATGATACTCCCATCC50.11462631820265932No Hit
GTACGAAGTGCTTCATTTCCTGTAAACTAGTTAATACATTGGCTGTGGTG50.11462631820265932No Hit
AGCACAGCTAAGTCGTCTGCAAAGGATTTGACCCCGCTCGTGTTTCTACC50.11462631820265932No Hit
TCCTATATATGTGTATGTAACTGGATGATGTCCTCTGGTCACAGTTTTAC50.11462631820265932No Hit
GTCCCAAAGGGGAGCTGTGGTCTTTTCTATGTGACCATATCTTCTGCCTA50.11462631820265932No Hit
TTCCTTGATAAAGTAAGATGTTTGCTTTTTCTCCCGGTTTTAGCTTTTCA50.11462631820265932No Hit
GCATAGTGGCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGG50.11462631820265932No Hit
TCCCAGAACAGAGTCAGCCAGACTTGTACCTACCAGACAGGTCACTGAAA50.11462631820265932No Hit
TAACAATACTGGCCTATTTCATCAAGTTTTATTTTGTCCTGCTTTTTCCC50.11462631820265932No Hit
GTCCCCAATGAAACCAGGAAACCAGACAGAGTTAACAAACACTACCATCT50.11462631820265932No Hit
GTCAAAGAACAGATATCGAACTACATAGAAAGTGAAACTGTGGGCCGGGC50.11462631820265932No Hit
GCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTC50.11462631820265932No Hit
ATATGACACCACTAGCAAGTTAGGGTTAAGGACCAATGAGATCGTGGAGT50.11462631820265932No Hit
ATTTTGTAGTGATGGAGGGCAGTTGAATAATTTTAAAAAGGGGAATTACA50.11462631820265932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA3407.842424E-610.661764145
Produced by FastQC (version 0.11.2)