FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_dakar-41671.351000000a1287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_dakar-41671.351000000a1287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154192
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAACCA3780.24514890526097333No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCCA2650.17186365051364533No Hit
GATATATATCAACAAGGGTTGAAATGGGCGAGGCGGCTGCTATCTTCATG2080.13489675210127633No Hit
ATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT2030.13165404171422643No Hit
CCATAAGTCCTCCCTTTTTCCCAAATAGGGAATGTCTGTCCATTTTGTTA1970.12776278924976653No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA1960.12711424717235653No Hit
GTATATAACTTTTTGGCTCGTCGAGCTTCCCAGGAGCCAGGCTATAGCTA1870.12127736847566671No Hit
CTCTATTCCACACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT1740.11284632146933693No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC1670.10830652692746705No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA1560.10117256407595726No Hit
GAGTTATGAGTGCCCCATGCTAGACGAGGGAGTGGAGCCAGATGACGTCG1550.10052402199854726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGT352.7505848E-7103.5714342
CGAGTTT352.7505848E-7103.5714344
GTTCGAG352.7505848E-7103.5714341
ATAATCA606.7784113E-660.4166688
TAATCAA606.7784113E-660.4166689
GGCTTTT502.0048904E-458.07
AGGATAA553.210453E-452.7272765
GATAATC553.210453E-452.7272767
CAGGATA553.210453E-452.7272764
CCTCTAA907.4529795E-540.27778145
ACATCGG750.00147949338.6666649
GTGTTAA800.00203128536.25145
GGGACAT850.002734830134.117656
TTGTATG900.00361879132.2222255
ATGAGGT950.004715033330.5263149
GTTGTAT950.004715033330.5263144
CCTAAGC1204.0422988E-430.2083342
ACCTAAG1204.0422988E-430.2083341
AATGGTT2651.364242E-1030.0943412
CCAGGCA1000.00605874629.02
Produced by FastQC (version 0.11.2)