FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_dakar-41667.351000000a127a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_dakar-41667.351000000a127a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174384
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAGCCA3750.21504266446462977No Hit
ATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT2750.15769795394072852No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCTA2290.13131938709973393No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA2080.11927699788971464No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC2070.11870355078447564No Hit
CTCTATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT2060.11813010367923663No Hit
CTATAAGGGATCCACACCATAAGTCCTCCCTTTTCCCCAAATAGGGAATG1950.11182218552160748No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA1880.1078080557849344No Hit
TCCGTACACTACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACGTCAT1860.10666116157445638No Hit
ACTCATTAGAGTGTGACCCAGCCGTCATAGGAACAGCTGTCAAGGGAAAG1850.10608771446921736No Hit
GAGTTATGAGTGCCCCATGCTAGACGAGGGAGTGGAGCCAGATGACGTCG1830.10494082025873934No Hit
CCTATGATCCTACGCACCATGTTGACTGTGTCTTTGATGTTCTCAGCCCA1810.1037939260482613No Hit
CCATAAGTCCTCCCTTTTCCCCAAATAGGGAATGTCTGTCCATTTTGTTA1750.10035324341682725No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCG100.007085878145.01
AGTTCGA100.007085878145.02
TGAGGTC203.860647E-4108.756
TGAAGCG502.2982513E-672.55
ATAGTCC607.992821E-872.58
TAGTCCG607.992821E-872.59
GGCTTTT754.82396E-967.6666647
GCTTTTT754.82396E-967.6666648
AATAGTC651.3912904E-766.923087
GAAGCGA451.19110584E-464.444446
AAGCGAG451.19110584E-464.444447
GAAATAG753.7430982E-758.05
AAATAGT753.7430982E-758.06
GCGAGAG553.2116243E-452.7272729
GATCGAC750.001480025838.6666647
TCGACTA750.001480025838.6666649
AAATAAG1157.108869E-637.8260885
TAAATAA1157.108869E-637.8260884
ATCTGAA1001.3867428E-436.252
TGTAAAT1251.2591185E-534.8000032
Produced by FastQC (version 0.11.2)