FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_472.351000000a1152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_472.351000000a1152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65883
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT5770.8757949698708316No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2310.35062155639542825No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1950.2959792359182187No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1690.2565153377957895No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1220.1851767527283214No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1030.15633775025423857No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1000.15178422354780444No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT990.1502663813123264No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT930.14115932789945815No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC930.14115932789945815No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA930.14115932789945815No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC860.13053443225111183No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT850.12901659001563376No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT820.12446306330919964No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT810.12294522107372159No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAACCTGAGACCAC790.11990953660276552No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTTCCTACATGCT780.11839169436728746No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG770.11687385213180942No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA760.11535600989633138No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC760.11535600989633138No Hit
GTTTTCAACTACTAATACAAACCCTCTTATTTGTATTCCAGCAGTGGCAA740.11232032542537529No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT740.11232032542537529No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT700.10624895648346311No Hit
GAACAAAATAGTACCAAATTGAGTACTATTTCCTTTTATTCATCCATTAT700.10624895648346311No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC690.10473111424798506No Hit
GTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTATTGGATGTTTAG670.10169542977702897No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA660.10017758754155093No Hit
CCATTCATTGAACCAGTATACACTCTGTATCACCCAAGGGTTGTTGCTAA660.10017758754155093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA100.007075935145.01
GTAACAA800.0126.8754
GAAACAC550.0118.636374
GAGCATC458.367351E-11112.777784
AAACACG600.0108.755
CAGAGCA501.9099389E-10101.52
TAGAAAC650.0100.384622
TAGTAAC950.099.210522
CGTTTTC301.5883345E-596.6666647
AGCATCT651.4551915E-1189.2307745
GCAGAGC950.083.9473651
AGTAACA1300.083.653853
ACGAGCC300.001927694272.54
TAACAAG1400.072.55
CGAGCCT300.001927694272.55
AAGTGGT300.001927694272.57
AACACGA851.6552804E-1068.23536
ACACGAG902.746674E-1064.444447
TTCGGAT902.746674E-1064.44444145
CCAAGCC350.003551730262.1428573
Produced by FastQC (version 0.11.2)