FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_468.351000000a1145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_468.351000000a1145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89668
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT4910.5475755007360485No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2790.3111477896239461No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2500.2788062631038944No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC2350.26207788731766074No Hit
GGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAG2150.23977338626934916No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1670.18624258375340144No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1640.1828969085961547No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT1600.1784360083864924No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1510.1683989829147522No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1370.1527858321809341No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1360.15167060712851851No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1210.13494223134228486No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1170.13048133113262256No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1160.129366106080207No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA1090.12155953071329795No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA1050.11709863050363564No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1030.11486818039880446No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1020.1137529553463889No Hit
GCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAGC1020.1137529553463889No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1010.11263773029397332No Hit
GGTGGATTAATACCTTTTTGCCAGGATATCATTGATTCATTAGACCGACC1000.11152250524155774No Hit
GGATACAACTTAAAACAAATGCTGAAGACATAGGAACCAAAGGCCAAATG990.11040728018914217No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT940.10483115492706428No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGCG100.0070801764144.999982
GAAGCGG100.0070801764144.999983
GCAGAAC100.0070801764144.99998145
TAGAAAC351.882654E-9124.285712
GAAACAC351.882654E-9124.285714
CGGTGCA301.0934673E-7120.8333362
CAGCCTT203.8544618E-4108.749995
GTAACAA1600.0104.218744
ACAAGAG1600.099.687497
TAGTAAC2000.083.3752
TATAACA353.4202007E-582.857142
GGTGCAT451.2237197E-680.555563
AAACACG554.34884E-879.0909045
AGTAACA2200.075.795453
TAACAAG2200.075.795455
ACAGCCT300.001929979472.54
GCGGTGC607.948438E-872.51
GAGCATC300.001929979472.54
GCTGATA300.001929979472.58
CTCAGAG300.001929979472.52
Produced by FastQC (version 0.11.2)