FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_463.351000000a1138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_463.351000000a1138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31713
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT1150.3626273137199256No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC820.2585690410872513No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT800.25226247910951344No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT670.21126982625421753No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC560.17658373537665942No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC530.16712389241005265No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT480.15135748746570807No Hit
GAATGGATGAAGAACACCCAGGTCTTTTTCAAGCAGCCTCACAGAATGCT410.12928452054362566No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA400.12613123955475672No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC400.12613123955475672No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT400.12613123955475672No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT400.12613123955475672No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT390.12297795856588781No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT380.11982467757701888No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC370.11667139658814998No Hit
GTTCACTTGCTTTGGGTGATTCCTCATACCCCCATTGATCAAAAAGGTCG350.11036483461041213No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA340.1072115536215432No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA340.1072115536215432No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT330.10405827263267431No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA330.10405827263267431No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC320.10090499164380538No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT320.10090499164380538No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGCA100.007058738144.999989
CGGTGCA100.007058738144.999982
CCAAGGC100.007058738144.99998145
TAGAAAC450.0128.888892
GAAACAC403.092282E-11126.874994
AAACACG403.092282E-11126.874995
AACACGA403.092282E-11126.874996
GTAACAA458.185452E-11112.777784
ACACGAG501.8735591E-10101.57
TAGTAAC501.8735591E-10101.52
ACAAGAG501.8735591E-10101.57
CACGAGC607.9489837E-1084.5833368
TAACAAG607.9489837E-1084.5833365
AGTAACA702.910383E-1182.857143
GTAGAAA755.2750693E-1177.333331
CAAGAGG502.2550666E-672.58
ACAGCAT553.9716906E-665.9090963
ACGAGCA553.9716906E-665.9090969
AACAAGA851.2520104E-859.7058836
GTAGTAA1006.82121E-1058.01
Produced by FastQC (version 0.11.2)