FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_460.351000000a112b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_460.351000000a112b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63168
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1660.26279128672745694No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1410.2232142857142857No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1240.19630192502532928No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1160.18363728470111448No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1140.1804711246200608No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT1120.1773049645390071No Hit
GTATATGCATACAATAGGATATTATTCATCCATAAAAGGGAATGAAGTAT1070.16938956433637287No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG1040.1646403242147923No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1030.16305724417426545No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1000.1583080040526849No Hit
GTCTATTGGAATTGAAGGGTTTGAGCCATACTGTATGAACAGTTCCTCAA1000.1583080040526849No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT990.15672492401215807No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA950.15039260385005065No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG950.15039260385005065No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT920.14564336372847012No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT910.14406028368794324No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG870.13772796352583586No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC860.136144883485309No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA860.136144883485309No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG840.13297872340425532No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG840.13297872340425532No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC780.12348024316109422No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC760.12031408308004053No Hit
GTCTAATATCTTCTTTAATTTTGAAGAATAGCTTTACTGGTGCAGAATTC730.11556484295845999No Hit
CTTTATATTACTTGAAGTTTACTGGGCTTCTTGGGTGCATAATGTTTTAA720.11398176291793312No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT710.11239868287740629No Hit
ATATAAAGAGATCCATATCTAGACACATTTAAATCAAAGTGTTGAAATAC710.11239868287740629No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA700.11081560283687944No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA680.10764944275582573No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG660.10448328267477203No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG640.10131712259371835No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC100.007075251145.04
CGCACTT100.007075251145.05
ACGAGCC100.007075251145.09
AACACGA100.007075251145.06
GTAACAA950.076.315794
TAGACCC300.001927326272.59
GCTAGGA300.001927326272.52
CTAGGAG300.001927326272.53
TAGTAAC1050.069.0476152
ACAAGAG1009.094947E-1265.257
TAACAAG1051.6370905E-1162.1428575
AGGAGAG350.00355105562.1428535
GAGCAGA350.00355105562.1428531
AGTAACA1301.8189894E-1255.769233
CAAGAGC1051.0804797E-955.2380948
ACTGCTC400.006024771454.3757
GAGAGAT400.006024771454.3757
GTAGAAA400.006024771454.3751
ACACTGC400.006024771454.3755
GAGATAC400.006024771454.3759
Produced by FastQC (version 0.11.2)