FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_451.351000000a111e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_451.351000000a111e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65073
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2360.3626696171991456No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1750.268928741567163No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1730.26585527023496686No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1590.24434097090959384No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1510.23204708558080928No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1420.21821646458592658No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1390.21360625758763235No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1270.19516542959445546No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1230.18901848693006315No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1210.18594501559786702No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG1090.16750418760469013No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1090.16750418760469013No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1090.16750418760469013No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1070.16443071627249398No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1020.1567470379420036No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG990.15213683094370936No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT990.15213683094370936No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC980.15060009527761128No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG960.14752662394541513No Hit
CTGCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAA950.14598988827931705No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC940.144453152613219No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA900.1383062099488267No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA890.13676947428272862No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC880.13523273861663054No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG850.13062253161833634No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT820.1260123246200421No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC810.12447558895394403No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA780.1198653819556498No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT740.11371843929125752No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG730.11218170362515945No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA700.10757149662686522No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA690.10603476096076714No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC680.10449802529466906No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA670.102961289628571No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA670.102961289628571No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT670.102961289628571No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG660.10142455396247292No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG660.10142455396247292No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA100.007075738145.07
ACGCACT450.0145.04
GTGGGGT100.007075738145.04
CACGCAC450.0145.03
GCACGCA450.0145.02
CGCACTT450.0145.05
AGCACGC450.0145.01
GCACTTC406.0530147E-790.6256
CGGTGCA259.3470607E-487.02
GTAACAA1500.087.04
TAGTAAC1650.079.090912
TAACAAG1950.066.923085
AGTAACA1950.066.923083
CAGAAGC350.00355153662.1428531
ACAAGAG2200.056.022737
GTAGTAA2600.055.769231
GGTGCAT400.006025585354.3753
GTGCCCG400.006025585354.3759
GCGGTGC553.1966437E-452.7272761
ACAGCAT553.1966437E-452.7272763
Produced by FastQC (version 0.11.2)