FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_450.351000000a1101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_450.351000000a1101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62309
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3200.5135694682951099No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2780.44616347558137665No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2710.4349291434624212No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC2280.36591824616026575No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2040.3274005360381326No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1910.3065367763886437No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1880.30172206262337703No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1850.2969073488581104No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1830.29369753968126594No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1830.29369753968126594No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC1770.28406811215073263No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG1730.27764849379704376No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1640.2632043525012438No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1510.24234059285175497No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC1490.23913078367491053No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA1470.2359209744980661No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA1460.23431606990964388No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1420.227896451555955No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1380.22147683320226613No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT1340.21505721484857726No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG1310.21024250108331058No Hit
CTGCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAA1280.20542778731804395No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT1250.2006130735527773No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG1240.19900816896435505No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA1160.18616893225697734No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG1080.17332969554959957No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCCTCAACACCAGA1070.17172479096117735No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT1070.17172479096117735No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG1040.1669100771959107No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA1030.16530517260748848No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA1030.16530517260748848No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC990.15888555425379963No Hit
CTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATAATGAT980.15728064966537741No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC980.15728064966537741No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG960.15407084048853295No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGA960.15407084048853295No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT950.15246593590011073No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT920.14765122213484408No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT920.14765122213484408No Hit
GTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTAC910.14604631754642186No Hit
GATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG910.14604631754642186No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT900.14444141295799964No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA900.14444141295799964No Hit
TTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCA900.14444141295799964No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG890.14283650836957743No Hit
GTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATA890.14283650836957743No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGACCACAA870.139626699192733No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA870.139626699192733No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC870.139626699192733No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT870.139626699192733No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG870.139626699192733No Hit
TATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTG860.13802179460431077No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC840.13481198542746634No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC840.13481198542746634No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAA820.1316021762506219No Hit
ATTTCATACAATGTTTCTAATAATGATAATGATACAACCATATCAGCTAT820.1316021762506219No Hit
ATCTATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAAT810.12999727166219968No Hit
AAATACAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGA800.12839236707377746No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA790.12678746248535525No Hit
ATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGA790.12678746248535525No Hit
ACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAG790.12678746248535525No Hit
CAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGACATAG780.12518255789693303No Hit
GGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGA770.1235776533085108No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA770.1235776533085108No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG760.12197274872008859No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAA760.12197274872008859No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT760.12197274872008859No Hit
GTTTGAGGAATGTTCCATTTACCAACACTCTCAAGGACAATACATTACGC750.12036784413166637No Hit
TGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGA750.12036784413166637No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC740.11876293954324416No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC740.11876293954324416No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC730.11715803495482194No Hit
ACATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTA730.11715803495482194No Hit
GTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGGTC720.11555313036639972No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA720.11555313036639972No Hit
GTTTTGTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAG720.11555313036639972No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA720.11555313036639972No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC720.11555313036639972No Hit
CCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATG710.1139482257779775No Hit
TTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC710.1139482257779775No Hit
GTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACAC700.11234332118955528No Hit
AGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGAC690.11073841660113307No Hit
ATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGC670.10752860742428863No Hit
GGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACCGCAGT670.10752860742428863No Hit
CCTATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTA670.10752860742428863No Hit
TATCAACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTT660.10592370283586641No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC660.10592370283586641No Hit
CATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTAA660.10592370283586641No Hit
TAATGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTT660.10592370283586641No Hit
GCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTG650.1043187982474442No Hit
GAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGAAGTAAACACTCAGA650.1043187982474442No Hit
GCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATC650.1043187982474442No Hit
CTCTGGTGTTGAGGGGTAATCGGTCCAATTGTATTTCGTACAGTTGCTAT640.10271389365902198No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT640.10271389365902198No Hit
GATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTACCAA640.10271389365902198No Hit
CACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGAT640.10271389365902198No Hit
ACTGAAATAGTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGAT630.10110898907059976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG100.007075025145.02
CGGTGCA151.2245192E-4145.02
GTGCGTT100.007075025145.04
GGTGCGT100.007075025145.03
CGTTTTC100.007075025145.07
GCAGAAG203.848877E-4108.752
GTAACAA1150.0100.869574
TAACAAG1250.092.85
TAGTAAC1550.074.838712
AAACACG300.001927203572.55
ACAAGAG1600.072.57
CAGAAGC300.001927203572.53
AGTAACA1750.066.285713
AGCATGC651.08172135E-555.7692347
GAAACAC400.00602439354.3754
GGTTCCC400.00602439354.3759
ATGTCAC400.00602439354.375145
AAGAGGA1405.456968E-1251.785719
GGCCGTC450.00959708848.3333327
GTTCTGC604.909221E-448.333332145
Produced by FastQC (version 0.11.2)