FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_445.351000000a10f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_445.351000000a10f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182099
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA7940.4360265569827402No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC4390.24107765556098606No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT3170.17408113169210154No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC2760.15156590645747642No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2470.13564050324274157No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT2420.1328947440677873No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA2380.13069813672782388No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2310.12685407388288789No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2220.12191170736797016No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2160.11861679635802502No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT2100.1153218853480799No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG2080.11422358167809818No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2010.11037951883316219No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT1860.10214224130829933No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA1830.10049478580332677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG800.0145.05
CAGAGGT100.0070861336145.02
CGGTGCA700.0124.285712
GAAACAC900.0104.722224
GTAACAA2150.0101.162794
AACACGA1050.089.76196
CGGAGCA353.429714E-582.857142
GCGGAGC353.429714E-582.857141
ACAAGAG2700.080.555567
ACGAGCA851.8189894E-1276.76479
GGATTGA1550.074.8387156
GAGCATC809.822543E-1172.54
TAGAAAC1450.070.02
ACACGAG1350.069.814827
TAACAAG3150.069.047625
AGCATCT955.456968E-1268.684215
AAACACG1450.065.05
AGAAGCG451.1912468E-464.444442
TAGAGTA451.1912468E-464.444445
CACGAGC1550.060.8064548
Produced by FastQC (version 0.11.2)