FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_443.351000000a10e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_443.351000000a10e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134033
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2660.19845858855654952No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2440.18204472033006797No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2100.15667783307096014No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2090.1559317481515746No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2080.1551856632321891No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1900.14175613468324966No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1610.12011967202106943No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1600.11937358710168391No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1470.10967448314967211No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1420.10594405855274447No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1410.10519797363335896No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1410.10519797363335896No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1400.10445188871397341No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1350.1007214641170458No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC1350.1007214641170458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAC100.0070840605145.03
GTGCTAC100.0070840605145.01
AGCACGC450.0128.888891
CGCACTT501.8189894E-12116.05
AGCATAG203.858675E-4108.755
GCACGCA553.6379788E-12105.454552
GGTGCAT352.748602E-7103.571433
GTAACAA2300.0100.869574
ACGCACT607.2759576E-1296.6666644
CACGCAC607.2759576E-1296.6666643
CGGTGCA406.0884304E-790.6252
TAACAAG2600.089.230775
TAGTAAC2650.087.547172
AACACGT259.368889E-487.06
AGTAACA2750.084.363633
ACGTGCA353.426402E-582.857142
ACACGTG300.001932072972.57
AGAAGGT300.001932072972.5145
GGGATAG300.001932072972.51
ACAAGAG3450.071.449277
Produced by FastQC (version 0.11.2)