FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_436.351000000a10d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_436.351000000a10d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137285
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2980.21706668609097862No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2790.20322686382343302No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2690.19594274684051424No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2360.1719051607968824No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG2150.15660851513275303No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2120.15442328003787742No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1870.13621298758058054No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1700.12382998870961869No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1690.1231015770113268No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1620.1180026951232837No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1620.1180026951232837No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG1550.11290381323524057No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1500.10926175474378118No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT1450.10561969625232182No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1440.10489128455402993No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA1390.10124922606257057No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1380.10052081436427869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT351.0913936E-11145.05
AGTAGGG100.007084246145.0145
CGCCATC100.007084246145.04
AGCACGC403.274181E-11126.8751
GCACGCA458.367351E-11112.777782
CGGTGCA607.2759576E-1296.6666642
ACGCACT554.129106E-1092.272724
TATTCTA259.369379E-487.05
CGGAGCA259.369379E-487.02
GTAACAA1750.082.857154
TAGTAAC1750.082.857152
CACGCAC702.7885108E-972.53
GAGCATC406.642741E-572.54
ACGAGCC607.9800884E-872.59
TAACAAG2300.063.043485
CCAGCGG350.00355995362.1428579
GCCAGCG350.00355995362.1428578
GGTGCAT851.294211E-859.705883
CTTTAAG502.004112E-458.01
AAACACG902.032175E-856.388895
Produced by FastQC (version 0.11.2)