FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_434.351000000a10cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_434.351000000a10cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101768
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3660.35964153761496737No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2260.22207373634148259No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA2170.21323009197390141No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1690.16606398868013522No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1620.15918559861646098No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1490.14641144564106595No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1410.13855042842543824No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG1380.13560254696957785No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1260.12381102114613632No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1250.12282839399418286No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1120.11005424101878784No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1080.10612373241097398No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG1050.10317585095511358No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1030.10121059665120667No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCA100.0070815715145.02
ACGCACT151.2267858E-4145.04
CACGCAC151.2267858E-4145.03
GCGGAGC151.2267858E-4145.01
GCACGCA151.2267858E-4145.02
AGCACGC151.2267858E-4145.01
CGCACTT151.2267858E-4145.05
GGAGCAT100.0070815715145.03
GAGCAGA256.436785E-6116.01
CGGTGCA256.436785E-6116.02
CAGCATC203.855975E-4108.754
AGAAGCG203.855975E-4108.755
AGCATCT750.0106.3333365
GAGCATC553.6379788E-12105.454554
GTAACAA1750.099.428574
TAGTAAC1950.089.230762
GAAGCGG259.36236E-487.06
AAACACG406.634486E-572.55
GCAGAGC809.822543E-1172.51
GCAGAAG406.634486E-572.53
Produced by FastQC (version 0.11.2)