FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_428.351000000a10bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_428.351000000a10bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60234
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT880.1460968888003453No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT770.12783477770030216No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC630.10459209084570176No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGG100.007074436145.03
ATACTAC100.007074436145.03
GTAGTAA950.091.578951
TACTACA259.3436445E-487.04
TAGTAAC1000.087.02
GTAACAA1200.072.54
GTATTAG350.003550247562.1428571
TAACAAG1500.058.05
GAGGATT1051.0786607E-955.2380944
GATTGGG1051.0786607E-955.2380947
ACGAGCT400.00602340954.3755
ATGTACT450.00959552548.3333365
AGTAACA1800.048.3333363
AAACACG604.9078895E-448.3333321
ACAAGAG1900.045.7894747
ATTGGGG1151.3831777E-744.1304368
AACAAGA2000.043.56
GGATTGG1505.18412E-1043.56
ACAACAG1851.8189894E-1243.108111
TATTAGA700.001048740341.428572
Produced by FastQC (version 0.11.2)