FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_425.351000000a10a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_425.351000000a10a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93638
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2410.2573741429761422No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2070.22106409790896858No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1870.1997052478694547No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1830.1954334778615519No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1530.16339520280228112No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1450.15485166278647558No Hit
CCTAAGGGGTAGCCACTACTCAGCACCCACTTTTTGTTGCCAGACCTTCC1410.1505798927785728No Hit
CATTTAGGTTTCTGGTCTCTGATAATTTGGAAGGTCTGGCAACAAAAAGT1390.1484440077746214No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1380.14737606527264574No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT1380.14737606527264574No Hit
GTAGAAACACGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC1330.14203635276276724No Hit
ATATTGTAGTTGGGTCTCTACAGTGAGGCAGGATCTGCCCAGGCAGAGGG1310.13990046775881587No Hit
CCCATATTCCAGGCCCTCCCACAGCCCTCACCCGGTCAGTCACCCCATCT1250.1334928127469617No Hit
GTAGTAACAAGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC1190.12708515773510756No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG1180.12601721523313186No Hit
CTACAGTGAGGCAGGATCTGCCCAGGCAGAGGGAAGAGCAGGCAGAGCCG1180.12601721523313186No Hit
CAGTAAAGTCTGAGCTAAAATCGGAGGCTCTCGAGCCACATCCACCTCGC1130.12067750272325337No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1090.11640573271535061No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGAAATAGTATGTTATCATC1070.11426984771139921No Hit
GGCTTAGATAAGTAGTTCCAATACCAGGTGCAATAGGGGAAGGAAAAAGT1040.11106602020547213No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG1040.11106602020547213No Hit
CCCCTTAGGTGGACCCTAAACTCTTCACTATAACTTCCCTGCCCAGCACC1040.11106602020547213No Hit
TCACAGAGCACCCCCTAGTCTTGGAAACCCTTCCTCAAAGAGTCACCTGG1030.10999807770349643No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1020.10893013520152076No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG1020.10893013520152076No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1010.10786219269954506No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG980.10465836519361797No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA950.1014545376876909No Hit
AATTAAAACAAGGGGTGATGGGAAGAATCACAGGTTGTGGCCCCGGGGTT950.1014545376876909No Hit
GTCTTGGGCAGGGCCTGAGAATGTGCGTTTCTAACCAGTTCCCAGGTGAT950.1014545376876909No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGG100.007080671145.02
GAGCATC202.120425E-6145.04
TACGTGG100.007080671145.08
GCACGCA203.8549985E-4108.752
ACGTGCG203.8549985E-4108.759
CGCACTT203.8549985E-4108.755
AAACACG502.2460881E-887.05
AACACGT259.3599956E-487.06
AGCACGC259.3599956E-487.01
AGCATCT353.420991E-582.857145
GTAGAAA1100.072.51
GAAACAC607.952258E-872.54
GTAACAA1200.072.54
TAACAAG1400.062.1428575
ATTAGTA606.757675E-660.4166682
TAGTAAC1450.060.02
CAAGAGC1350.059.0740748
ATATAAC502.0008018E-458.01
CACGTGC400.006033832754.3758
TAGAAAC1353.6379788E-1253.7037052
Produced by FastQC (version 0.11.2)