FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_421.351000000a1096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_421.351000000a1096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57791
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT1330.23013964112058974No Hit
GTTATAACCTGTGTTTGAGAAAGGCTAGCTCTGTTTCTATTGCCATAATT1150.19899292277344222No Hit
ACAACAGCCAACATAGATACCACAGGGCACAAAAGGCCAGGAATACAAAA980.16957657766780296No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT750.1297779931131145No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG680.11766538042255716No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC650.11247426069803255No Hit
TCTGTATACCCTCTAATGAAAATTTTTTAAAGTACCAAGAAATTAAAAAG650.11247426069803255No Hit
GTGCTACTCCTGTGACACGCAATGTCTTCTAGCTTGTTGGTTCCACAGTT620.10728314097350798No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA620.10728314097350798No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAT203.8474356E-4108.754
CGCACTT501.9099389E-10101.55
TAGTAAC950.099.210532
GTAACAA900.096.6666644
GCACGCA554.0745363E-1092.2727362
AGCACGC554.0745363E-1092.2727361
TAACAAG1050.089.761915
GTAGTAA1050.089.761911
CCTATAG259.341702E-487.09
CTAGGAC259.341702E-487.03
TTCTGCG353.409868E-582.857147
GCACTTG353.409868E-582.857146
ATAGAGG406.610208E-572.59
CTGCGCA406.610208E-572.59
GTTGTAA502.278799E-672.51
GGACCTC300.001926487572.56
ACGAGCA502.278799E-672.53
ACGCACT754.7475623E-967.666674
TCTGCGC451.1843933E-464.444448
GTTATTC451.1843933E-464.444443