FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_420.351000000a1089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_420.351000000a1089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118780
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT2270.19110961441320087No Hit
GCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT2140.18016501094460347No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA2000.16837851490149855No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1960.1650109446034686No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1760.14817309311331875No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1700.14312173766627379No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1530.1288095638996464No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1480.12460010102710893No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1350.11365549755851154No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGAT100.007083052145.01
CGGTGCA600.0120.8333362
GGTGCAT458.367351E-11112.777783
ACGTGCA203.8575806E-4108.752
GCGGTGC800.090.6251
GAAACAC651.4551915E-1189.2307744
AAACACG651.4551915E-1189.2307745
AACACGA755.4569682E-1177.3333366
AGCGGTG300.001931529772.58
TAACAAG2450.065.102045
ACGAGCC451.1895469E-464.444449
ACACGAG954.5110937E-1061.0526287
TAGTAAC3000.058.0000042
GTAGTAA3250.058.01
GAGCATC1258.731149E-1152.2000054
GTAACAA3200.052.1093754
TATTCGG1700.051.176468145
GCAGAGC1301.2914825E-1050.192311
ACAAGAG3250.049.0769237
CACGAGC1203.5743142E-948.3333328
Produced by FastQC (version 0.11.2)