FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_418.351000000a107c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_418.351000000a107c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304064
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA7840.2578404546411282No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT7010.23054356977478424No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG6270.20620658808671857No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC5070.16674121237634182No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC4850.15950589349610608No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG4840.15917701536518628No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA4810.15819038097242685No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT4690.1542438434013892No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG4100.1348400336771206No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT3970.13056461797516314No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT3950.12990686171332352No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA3870.12727583666596506No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3850.12661808040412545No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG3820.12563144601136603No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG3710.12201378657124816No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG3550.11675173647653127No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA3480.1144495895600926No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC3360.11050305198905494No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT3340.10984529572721533No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG3320.10918753946537571No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA3270.10754314881077669No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC3260.10721427067985688No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA3190.10491212376341824No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT3120.10260997684697958No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA3080.10129446432330036No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC3050.10030782993054094No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT3050.10030782993054094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG100.007088453145.0145
CGCACTT1000.0123.255
AGCACGC1100.0112.045451
CGGTGCA501.9463187E-10101.52
GTAACAA4650.091.989254
ACGCACT1350.091.2962954
GCACGCA1400.088.035722
TAGTAAC5350.079.953272
AGCATCT1650.074.696975
ACACGTG300.001934442372.52
AACACGA1350.069.814826
TAACAAG6150.069.552845
AGTAACA6450.066.3178253
AAACACG1650.065.909095
GCACTTC902.8194336E-1064.444446
GAAACAC1750.062.1428574
CACGCAC2000.061.6253
GAGCATC1700.059.705884
ACAAGAG7450.057.4161077
CGGAAAA651.0918249E-555.7692341
Produced by FastQC (version 0.11.2)