FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_412.351000000a106f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_412.351000000a106f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157015
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG5590.356016941056587No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG2540.16176798395057798No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG2440.1553991656848072No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2410.15348852020507595No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT2240.1426615291532656No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT2160.13756647454064896No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1970.12546571983568447No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1960.1248288380091074No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1900.12100754704964495No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG1860.11846001974333661No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1780.11336496513072No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1750.11145431965098876No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC1700.10826991051810338No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC1700.10826991051810338No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC1690.1076330286915263No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT1670.10635926503837212No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1670.10635926503837212No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT1650.10508550138521797No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1640.10444861955864089No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1590.10126421042575551No Hit
GCACAGCATTTTCTTGTGAACTTCAAGTACCAGTAAAAGAACTGAAAATC1590.10126421042575551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA650.0122.6923142
GCGGTGC900.096.6666641
GGTGCAT850.093.823533
GTAACAA2750.073.8181844
GAAGCGG300.001932693872.51
GAAACAC607.987546E-872.54
GCGGAGC300.001932693872.51
CGTTTGA406.645719E-572.52
TAAGATG300.001932693872.56
CAAGAGG1850.066.621638
TAACAAG3050.066.557385
TTTAAGA350.003560907762.1428574
GCAGAGC1550.060.806451
GCACAGC1102.5465852E-1159.318181
CAGAAGC502.0050051E-458.03
AAACACG805.8417027E-754.3755
AACACGA1101.6607373E-952.7272721
TAGTAAC4000.050.752
ACACGAG1152.4610927E-950.434782
ACAAGAG4150.048.915667
Produced by FastQC (version 0.11.2)