FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_403.351000000a1039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_403.351000000a1039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91487
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1990.21751724288696755No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1550.16942297812804005No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1300.14209669133319489No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1260.13772448544601965No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1100.12023566189731875No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1060.11586345601014353No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1050.11477040453834972No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT1050.11477040453834972No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1030.11258430159476208No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1000.10930514717938068No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG980.10711904423579305No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC970.10602599276399927No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA970.10602599276399927No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC950.10383988982041165No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC930.10165378687682404No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA100.0070804064145.02
GTAACAA850.0102.352944
GAAACAC502.2455424E-887.04
AAACACG502.2455424E-887.05
ACGAGCC353.420569E-582.857149
TAGTAAC1300.066.923082
CTTTAGC350.003556152862.1428572
ACTTTAG350.003556152862.1428571
TAACAAG1450.060.05
ACACGAG753.7232894E-758.07
CATAACC502.0005574E-458.06
ACAAGAG1500.058.07
AACACGA753.7232894E-758.06
ACTGATC400.006033390854.3758
CGAGCAT400.006033390854.3753
TAGCTCT400.006033390854.3755
AATGTAA400.006033390854.375145
TAGAAAC953.097375E-853.4210472
GTAGAAA953.097375E-853.4210471
CAGAAGC701.6837928E-551.7857133
Produced by FastQC (version 0.11.2)