FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_402.351000000a102c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_402.351000000a102c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137254
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2620.19088696868579422No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2140.15591531030061057No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2010.14644381948795665No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1900.1384294811080187No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1810.1318722951607968No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1630.11875792326635289No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG1570.11438646596820493No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC1520.11074358488641498No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1520.11074358488641498No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1510.11001500867005698No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1480.107829280020983No Hit
AGCACAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAAC1480.107829280020983No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1450.10564355137190902No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1440.10491497515555102No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1420.10345782272283502No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1410.10272924650647705No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGATT151.2277123E-4145.05
ACGACTG100.007084246145.0145
CTATTAC301.09659595E-7120.8333363
GAAACAC550.0118.636364
CGGTGCA203.8588763E-4108.752
GGTGCAT203.8588763E-4108.753
GAGCAGA404.783942E-9108.751
AAACACG650.0100.384625
AACACGA650.0100.384626
GTAACAA950.099.210534
AGAAGCG451.0846634E-896.6666645
TAGAAAC850.085.294112
ACACGAG801.8189894E-1281.56257
CAGCCCT451.2272067E-680.555569
GAAGCGG554.3661203E-879.0909046
CACGAGC851.8189894E-1276.76478
CACAGGA300.001932173572.53
ACGAGCC502.2956392E-672.59
TACACCG300.001932173572.55
TAACAAG1450.065.05
Produced by FastQC (version 0.11.2)