FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_398.351000000a101f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_398.351000000a101f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5782
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCAATGCAATCTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTT170.2940159114493255No Hit
GGCTAACCTTCTCAAAACGCACTCATAATGAGCAGATGTTATCATTCTTT150.2594258042199931No Hit
TCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTGCATTTGCTTT150.2594258042199931No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA140.24213075060532688No Hit
GTTTACAGCAGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACA140.24213075060532688No Hit
AGCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTT140.24213075060532688No Hit
GCCTTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTG130.2248356969906607No Hit
GCTTTCAATGGAAATTTTAAACAAGGGGGATTAAGATCCTGAAGCACTTC130.2248356969906607No Hit
GTCAAGAGCAAAGATCATGGCAACATTTTGGGATGCTCAAGGCATTTTGC120.20754064337599448No Hit
AATTAAAGTTGTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGT110.19024558976132827No Hit
CATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACA110.19024558976132827No Hit
GAATAAGCAGGAGTATTGCTGTGGTGGAAAAGGACCCTCTGGTGAAGCTT110.19024558976132827No Hit
GCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAA110.19024558976132827No Hit
CTCATAATGAGCAGATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAA110.19024558976132827No Hit
GGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAA100.17295053614666206No Hit
GCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAAT100.17295053614666206No Hit
TTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGA100.17295053614666206No Hit
CTTTTATAAGTGGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAG100.17295053614666206No Hit
GTGTTACTCTGCTCATGCTCTGGCTAACCTTCTCAAAACGCACTCATAAT100.17295053614666206No Hit
TTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACACC100.17295053614666206No Hit
AGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAA90.15565548253199585No Hit
GGACATGGCTTTACCAGTATGATTCTGAAGACAAAGCACAATCAAAGCAA90.15565548253199585No Hit
GTCAGAAGGAGACAAATCAGGACTGAAAGGTGGATGCCCAATGATTTCCC90.15565548253199585No Hit
GAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGAAAAGTTGCCT90.15565548253199585No Hit
ATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTGATGA90.15565548253199585No Hit
AGTAGAAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACA90.15565548253199585No Hit
TCATTATGTAATTCAGTTTTTCCACAAGTTTTCCCTCTCTCCTCACTGCT90.15565548253199585No Hit
TTATCATTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTGAGCA90.15565548253199585No Hit
CTTCTTGGAAGAACTGCAATAGGACATGGCTTTACCAGTATGATTCTGAA90.15565548253199585No Hit
CATCTGCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCA90.15565548253199585No Hit
TCTTGGAAGAACTGCAATAGGACATGGCTTTACCAGTATGATTCTGAAGA80.13836042891732964No Hit
GAATTACATAATGAACATAAAAAATGTGAACTTTATTTTTTTAACTTAAG80.13836042891732964No Hit
CTGCTAGGATGTCCCCCTTGTCCATGTTATCCAGGATGGCTCACCACCAT80.13836042891732964No Hit
AGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACATAATGAACA80.13836042891732964No Hit
GTCTTGGCTAAGGGAAGCCCAGGGAAAGGTTTTGATCTTTACTTCAGCCA80.13836042891732964No Hit
GTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGTCCAGCTCAGC80.13836042891732964No Hit
GTGTAGGAGATTAACCCCATAACTTGGTTTCTTATTTAAAAATTTCTTGA80.13836042891732964No Hit
GAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGAAACAAGAAG80.13836042891732964No Hit
TTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAA80.13836042891732964No Hit
CCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTAAA80.13836042891732964No Hit
TGCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAA80.13836042891732964No Hit
ATTTTGGGATGCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAA80.13836042891732964No Hit
GGCTTTACCAGTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTA80.13836042891732964No Hit
TCATTGGGCATCCACCTTTCAGTCCTGATTTGTCTCCTTCTGACTTCTTG80.13836042891732964No Hit
GTATAAGGACAATACCTCACCTGAACTACTACTGCAGGTTGTTCCTGGAA70.12106537530266344No Hit
TTACAGCAGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACATA70.12106537530266344No Hit
GCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATGCAGACCAGTCAA70.12106537530266344No Hit
ATCATTGGGCATCCACCTTTCAGTCCTGATTTGTCTCCTTCTGACTTCTT70.12106537530266344No Hit
GGTAAAGCCATGTCCTATTGCAGTTCTTCCAAGAAGTGCTTCAGGATCTT70.12106537530266344No Hit
AAGCAATGGCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATGCAGA70.12106537530266344No Hit
CTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTT70.12106537530266344No Hit
GTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAAT70.12106537530266344No Hit
GCTCATGCTCTGGCTAACCTTCTCAAAACGCACTCATAATGAGCAGATGT70.12106537530266344No Hit
GTAGAAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAA70.12106537530266344No Hit
GCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTG70.12106537530266344No Hit
GTAGAAACAACAGCCGCCAGCAGAAGAGATGCTACAAGCATCACCCACGT70.12106537530266344No Hit
GAATTTAACCATGTCAATGCAATCTTTTTTACATTATTAACTGAAGAAAA70.12106537530266344No Hit
AATCAGGACTGAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGA70.12106537530266344No Hit
GCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTA70.12106537530266344No Hit
GATTAAACATAAATGGTTCTGAAATAACAAAATTTTATTTAAAAATGGGA60.10377032168799724No Hit
GTAGAAACAACAGCAGCAGTAACAAAAACCAACAGTGGCTTAAACAAGAT60.10377032168799724No Hit
CTTTAAAGCCATCAGATCTCAGACTTATTCAGTATCGGGAGAACAGTACA60.10377032168799724No Hit
CTGGTGAAGCTTTCCCAGGTGTTACTCTGCTCATGCTCTGGCTAACCTTC60.10377032168799724No Hit
GTCCTATTGCAGTTCTTCCAAGAAGTGCTTCAGGATCTTAATCCCCCTTG60.10377032168799724No Hit
TCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTGGAAATAG60.10377032168799724No Hit
GTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTG60.10377032168799724No Hit
GAAGGAGACAAATCAGGACTGAAAGGTGGATGCCCAATGATTTCCCATCA60.10377032168799724No Hit
AACAAGAAGTCAGAAGGAGACAAATCAGGACTGAAAGGTGGATGCCCAAT60.10377032168799724No Hit
CCTTTACAGATTTTTTAAGATTAGGAAACAAGAAGTCAGAAGGAGACAAA60.10377032168799724No Hit
GTCAAAGCAAATGCAGACCAGTCAAGAGCAAAGATCATGGCAACATTTTG60.10377032168799724No Hit
GTTATATTAGTGAGAGACAGAAAGAGAGAACAGACACTAGAGTCTCTAGC60.10377032168799724No Hit
CTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTGAGCATCCCAAAA60.10377032168799724No Hit
ATCAAAGCAATGGCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATG60.10377032168799724No Hit
ATTCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTGGAAAT60.10377032168799724No Hit
TTCCACCACAGCAATACTCCTGCTTATTCTCATCAAATGTAGGCAACTTT60.10377032168799724No Hit
GTCCAGCTCAGCGGCAGACAAGAGCACAGCTTTCAATGGAAATTTTAAAC60.10377032168799724No Hit
GGTTACACGTTTCTTGTATTTTCTATGTTTATGCTCTTCTTTGTCTTCTC60.10377032168799724No Hit
CCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAATTTAACCATGTCAA60.10377032168799724No Hit
GCAATACTCCTGCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTT60.10377032168799724No Hit
GTTCTGAAATAACAAAATTTTATTTAAAAATGGGAAAGATTAGTTTACAG60.10377032168799724No Hit
ATTTCCACCTCTTGGTAGCCATTGCTTTGATTGTGCTTTGTCTTCAGAAT60.10377032168799724No Hit
GCATTGACATGGTTAAATTCCCAGGACCCTTAGTTTTTTAGGGATGAATT60.10377032168799724No Hit
GCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAAC60.10377032168799724No Hit
GAATAAACAAAAGGTAAATTGTTGGACTTAAGCCCTAACACATAGGTAAT60.10377032168799724No Hit
TCCAAGAAGTGCTTCAGGATCTTAATCCCCCTTGTTTAAAATTTCCATTG60.10377032168799724No Hit
GGAAACAAGAAGTCAGAAGGAGACAAATCAGGACTGAAAGGTGGATGCCC60.10377032168799724No Hit
ATATTAGTGAGAGACAGAAAGAGAGAACAGACACTAGAGTCTCTAGCAGT60.10377032168799724No Hit
CATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAATCATTGGGCA60.10377032168799724No Hit
CGTGTAACCTTCCCAGCTTACAAAGTCCAAGATCTCTATCAGCTCTTCCT60.10377032168799724No Hit
ATCTTTGCTCTTGACTGGTCTGCATTTGCTTTGACTGGGCTATTTCCACC60.10377032168799724No Hit
GATAACATCTGCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCAT60.10377032168799724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAGCT100.0069098407145.04
ACGAGCC258.9159096E-487.09
ACACGAG300.001838992972.57
AAACACG300.001838992972.55
CACGAGC300.001838992972.58
AACACGA300.001838992972.56
GAAACAC350.003388886862.1428574
GTAGAAA450.00916253948.3333321
Produced by FastQC (version 0.11.2)