FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_395.351000000a1002.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_395.351000000a1002.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156888
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3090.19695579011779102No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2800.1784712661261537No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2310.1472387945540768No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2070.13194125745754934No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2040.1300290653204834No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1980.12620468104635152No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1890.12046810463515374No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1860.11855591249808782No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA1830.11664372036102187No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1800.11473152822395595No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1790.11409413084493396No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT1710.10899495181275815No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1700.10835755443373617No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1690.10772015705471419No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACT202.1249507E-6145.04
CACGCAC202.1249507E-6145.03
GGTGAGT100.007085204145.09
CGCACTT202.1249507E-6145.05
GTAACAA1700.0123.676474
GCACGCA256.447759E-6116.02
AGCACGC256.447759E-6116.01
AAACACG800.0108.755
CAGAGCG203.859916E-4108.753
TAGAAAC850.0102.3529362
GAAACAC900.096.6666644
GCGGAGC750.096.6666641
TAACAAG2400.087.6041645
AGCGTTT259.3718915E-487.06
CCAGCCA259.3718915E-487.08
GAGCGTT259.3718915E-487.05
CGGTGCA353.42823E-582.857142
ACAAGAG2550.082.450997
TAGTAAC2650.079.339622
CGGAGCA554.370304E-879.090912
Produced by FastQC (version 0.11.2)