FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_394.351000000a0ff7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_394.351000000a0ff7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184898
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT4780.25852091423379375No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT4720.2552758818375537No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG4460.24121407478718No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA4340.23472400999469978No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC3960.21417213815184588No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG3480.18821187898192518No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT3340.18064013672403162No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA3310.17901762052591158No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC3190.1725275557334314No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT2770.14981232895975077No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA2700.146026457830804No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC2610.1411589092364439No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG2560.13845471557291045No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG2460.13304632824584364No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC2440.1319646507804303No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG2350.12709710218607015No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT2310.12493374725524344No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA2290.12385206978983007No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT2260.12222955359171002No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG2260.12222955359171002No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA2230.12060703739359No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA2210.11952535992817662No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA2090.11303529513569643No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT2090.11303529513569643No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG2080.11249445640298976No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG2050.1108719402048697No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA2010.108708585274043No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC2000.1081677465413363No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC1970.10654523034321625No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCTTCAACACCAGA1940.10492271414509621No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT1920.10384103667968286No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT1920.10384103667968286No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA1890.10221852048156281No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT1850.10005516555073607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA1650.087.8787844
TAACAAG1750.082.857155
CAAACGT451.191295E-464.444444
GTTAACC350.003561907762.1428579
TAGTAAC2350.061.702132
ACGTTAA400.00604311654.3757
ACACCAC400.00604311654.3758
TAAGTTT400.00604311654.3755
TACACCA400.00604311654.3757
ACAAGAG2900.050.07
GGATCAC450.00962680548.3333329
TTATATA450.00962680548.3333322
GAGCAGA1255.156835E-946.41
AGTAACA3300.046.136363
GTGCCCG700.001054405541.428579
CAGAAGC1451.913213E-840.04
AAGAGCG750.001480256738.6666649
TTATGAG750.001480256738.6666643
CTATTAC750.001480256738.6666641
GTAGTAA4600.034.6739161
Produced by FastQC (version 0.11.2)