FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_390.351000000a0fea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_390.351000000a0fea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129523
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2210.17062606641291508No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT1740.13433907491333585No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1670.12893462937084535No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1600.12353018382835482No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC1510.11658161098800986No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1460.11272129274337377No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1460.11272129274337377No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1380.10654478355195601No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC1350.10422859260517438No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1320.10191240165839272No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTT100.007083787145.05
ACGTGCC100.007083787145.09
ACAGCGT100.007083787145.04
CACAGCG100.007083787145.03
CACGTGC100.007083787145.08
GTTATTA100.007083787145.02
AAACACG406.087266E-790.6255
AGCGTTT259.368174E-487.06
GTAACAA1400.082.857154
GAAGCGG451.1899524E-464.444456
AGAAGCG451.1899524E-464.444455
TAACAAG1800.064.444455
GCGGAGC451.1899524E-464.444451
AACACGA350.003559497962.142866
GAAACAC606.772305E-660.4166684
GAGCAGA502.0036868E-458.01
TAGTAAC2050.056.5853652
GAGCATC651.0883075E-555.7692344
ACAAGAG2100.055.2380947
TAGAAAC805.833663E-754.3752
Produced by FastQC (version 0.11.2)