FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_385.351000000a0fdd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_385.351000000a0fdd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA3240.3099084622227324No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG3230.3089519546232795No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG2920.27930021904024027No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT2680.2563440366533712No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC2610.24964848345720106No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2550.24390943786048383No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2540.24295293026103093No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC2530.24199642266157806No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG2390.22860531626923775No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA2200.21043167187963308No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2180.2085186566807273No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA1990.19034501229112263No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG1980.1893885046916698No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC1960.18747548949276402No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA1900.18173644389604676No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT1890.18077993629659386No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT1820.17408438310042373No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1810.17312787550097086No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT1790.1712148603020651No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT1790.1712148603020651No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG1770.16930184510315935No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT1770.16930184510315935No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA1730.16547581470534783No Hit
CTTCAAGGGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT1630.15591073871081906No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC1570.1501716931141018No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA1560.1492151855146489No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCTTCAACACCAGA1540.14730217031574316No Hit
GTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATGACCACA1530.1463456627162903No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA1530.1463456627162903No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA1520.14538915511683742No Hit
GAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGATCTTACAGTGG1430.13678058672176152No Hit
GTTTAGGCGGTCTTGACCGGGGTAGTCAAGGGCTCTTTGCCATGAAAGCC1420.13582407912230862No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG1390.13295455632394998No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTA1390.13295455632394998No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC1370.13104154112504424No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT1360.13008503352559136No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG1350.1291285259261385No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1300.1243459879288741No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACA1300.1243459879288741No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC1260.12051995753106258No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC1250.11956344993160971No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA1240.11860694233215682No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTTATTCTTGACTCT1240.11860694233215682No Hit
GAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGAAGTAAACACTCAGA1230.11765043473270397No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG1220.11669392713325108No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA1220.11669392713325108No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC1210.1157374195337982No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA1200.11478091193434531No Hit
ACATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTA1200.11478091193434531No Hit
CTTCTGGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACC1190.11382440433489244No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC1180.11286789673543958No Hit
CAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGACATAG1170.1119113891359867No Hit
GCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTG1150.10999837393708092No Hit
GGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACCGCAGT1130.10808535873817518No Hit
ACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAGTGCTATGAAAG1130.10808535873817518No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG1120.10712885113872231No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT1120.10712885113872231No Hit
ATTTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGA1110.10617234353926942No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG1110.10617234353926942No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCG1100.10521583593981654No Hit
CTGTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT1090.10425932834036365No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG1090.10425932834036365No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC1080.1033028207409108No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA1060.10138980554200504No Hit
CTCTGGTGTTGAAGGGTAATCGGTCCAATTGTATTTCGTACAGTTGCTAT1060.10138980554200504No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT1060.10138980554200504No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG1060.10138980554200504No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA1050.10043329794255215No Hit
ATTGTATTTCGTACAGTTGCTATTTGAGGAACTGTTCATACAGTATGGCT1050.10043329794255215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG259.363072E-487.03
CACGGGT259.363072E-487.04
TATCTAG259.363072E-487.06
GTAGAAA903.6379788E-1272.51
CGTAGAG406.6353256E-572.53
GAACACG300.001930878472.51
CAGAAGG350.00355757562.1428579
ATATCTA350.00355757562.1428575
GTTCCCT350.00355757562.1428579
GGATACC606.763179E-660.4166689
TAAATAC502.0018869E-458.04
TTGTACA400.006035793654.3754
ACGGGTT400.006035793654.3755
TCTATAC400.006035793654.3753
ACACGAG553.205658E-452.7272767
TCATAGA553.205658E-452.7272763
CTTCATA858.8474735E-751.176471
ACTGTCC858.8474735E-751.176478
GCATACT858.8474735E-751.176474
TAGTAAC1457.2759576E-1250.02
Produced by FastQC (version 0.11.2)