FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_383.351000000a0fc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_383.351000000a0fc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138234
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC3140.22715106269079965No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2180.1577036040337399No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1990.14395879450786347No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1760.12732034087127625No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1620.11719258648378837No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1600.11574576442843294No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1540.1114052982623667No Hit
GGTCAGTACAGTGGGTTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA1530.11068188723468901No Hit
ATCATATGTTATCATCAGCTGTGCTTGTGATTCTAATTCACTCACGTCAT1390.10055413284720113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA1050.089.76194
AAACACG554.366302E-879.0909045
ACGAGCA502.2957247E-672.59
AACACGA607.980452E-872.56
CTATATA406.642906E-572.499991
ACACGAG651.3891622E-766.923087
CACGAGC651.3891622E-766.923088
TAGTAAC1500.062.8333362
GAAACAC702.3197208E-762.1428574
TAACAAG1750.053.857145
ACAAGAG1750.053.857147
TAGAAAC1004.663707E-850.752
CAGAGCA450.00962170248.3333322
CCACCCT450.00962170248.3333329
GTATATG1002.7169917E-643.51
AGTAACA2250.041.888893
GTAGAAA1655.9530066E-835.1515161
GACCGCC1251.2572324E-534.87
AAGACCG1251.2572324E-534.85
AGACCGC1251.2572324E-534.86