FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_380.351000000a0fb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_380.351000000a0fb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65171
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA1070.16418345583158153No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT860.1319605345936076No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC820.12582283531018398No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT800.12275398566847218No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG800.12275398566847218No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT770.11815071120590447No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG760.11661628638504856No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAA100.007075764144.999982
CAGGCGC100.007075764144.999986
CGGTGCA259.347125E-487.02
GGTGCAT300.001927601672.53
GTAACAA1400.072.54
GAAGCGG406.61658E-572.499991
TAACAAG1500.067.666675
GCGGTGC451.18553144E-464.444441
CGAGCAT451.18553144E-464.444443
AACACGA451.18553144E-464.444441
AAGCGGT350.003551560562.1428572
AGCGGTG350.003551560562.1428573
GTAGTAA1700.059.705881
ACACGAG501.9962639E-458.02
ACAAGAG1750.058.07
TAGTAAC1750.058.02
GCAGAGC651.0822754E-555.7692341
AGTAACA1850.054.864863
CGTGGCA400.00602562854.3749964
CCGTGGC400.00602562854.3749963