FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_379.351000000a0f9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_379.351000000a0f9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139110
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2760.19840414060815184No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2540.18258931780605275No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1980.1423334052188915No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1810.13011286032636044No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC1720.1236431600891381No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1700.12220544892531092No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1680.12076773776148372No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1670.12004888217957012No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT1660.11933002659765653No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1580.11357918194234778No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1530.10998490403277981No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT1530.10998490403277981No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1520.10926604845086622No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1420.1020774926317303No Hit
GTATTTAGGATTGGCTCCCTATTTGTGAATGGGAGGGAAAAATCTGTGTA1420.1020774926317303No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1420.1020774926317303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC450.0128.888894
AAACACG450.0128.888895
GTAACAA1450.0125.04
CGGTGCA352.749166E-7103.571432
GAGCAGA301.5945992E-596.6666641
TAACAAG1900.095.394745
GGTGCAT406.089649E-790.6253
AAGCGGA259.3696464E-487.02
TAGTAAC2350.077.127662
TAGAAAC903.6379788E-1272.52
CGGAGCA406.643057E-572.52
AGAAGCG300.001932228772.55
AGCGGAG300.001932228772.53
AACACGA955.456968E-1268.684211
ACAAGAG2650.068.3962257
GCGGAGC554.043264E-665.9090961
ACGAGCC451.1902618E-464.444449
CAGAAGC451.1902618E-464.444444
AGTAACA2850.063.5964933
CAGAGCA1150.063.043482
Produced by FastQC (version 0.11.2)