FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_377.351000000a0f8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_377.351000000a0f8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC1240.11627906976744186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT351.886292E-9124.285715
GTAATAC259.363595E-486.999993
ACGCACT651.546141E-978.076924
GCACGCA651.546141E-978.076922
AGTAGTA300.001930985172.54
GTAGTAA754.800313E-967.6666641
AGCACGC754.800313E-967.6666641
AACACGA451.1889902E-464.444441
TAGTAAC807.994458E-963.43752
CACGCAC1004.6502464E-850.7499963
AAGCGGA450.00961571148.3333324
TAACAAG1109.847463E-846.1363685
AGAAGCG657.307014E-444.6153832
CACGAGC657.307014E-444.6153833
AAACACG657.307014E-444.6153835
GCACTTA657.307014E-444.6153836
GTAACAA1201.9513573E-742.2916684
GAAGCGG700.001052394641.428573
AGTAACA1553.4207915E-837.4193533
ACACGAG800.002028477836.252
Produced by FastQC (version 0.11.2)