FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_373.351000000a0f70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_373.351000000a0f70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285547
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT3690.1292256616248814No Hit
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC3500.12257176576885767No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3080.10786315387659474No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG100.007088227145.06
ACACGAG1550.065.483877
CGGTGCA451.19240314E-464.444442
ATTAACG350.003563895462.142853145
AAACACG1850.058.7837875
CACGAGC1800.052.361118
GTAGGAT450.00963213848.3333323
ACGGGTG450.00963213848.3333325
GTAAGCT450.00963213848.3333323
ACGAGCC604.9391063E-448.3333329
AACACGA2000.047.1256
GCACGCA657.327744E-444.6153872
TTATAGC657.327744E-444.6153873
AGCACGC700.001055372141.428571
GGGCCGT1105.251086E-639.5454526
TGGGCCG1105.251086E-639.5454525
ACGAGCA1502.588422E-838.6666649
GAAACAC2850.038.1578944
ACGCTCG1901.2732926E-1038.1578949
TAAGACG1901.2732926E-1038.1578945
Produced by FastQC (version 0.11.2)