FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_369.351000000a0f63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_369.351000000a0f63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137475
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1680.12220403709765411No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC1620.11783960720130934No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1570.11420258228768868No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC1410.10256410256410256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTG100.0070842574145.05
GCATGAT100.0070842574145.02
ACCCAGT203.8588885E-4108.758
TATATAC259.369408E-487.03
TGTATCG300.001932179672.55
GGATTGC406.642776E-572.59
TATCGCA300.001932179672.57
GTGTAGT451.1902116E-464.444451
GTATCGC350.00355996362.142866
GTGCCCG858.868119E-751.1764689
TCGCATG450.00962159148.3333369
TTGTGTA450.00962159148.3333362
GTCACTA450.00962159148.333336145
ACTACAC752.539018E-548.3333363
GCAGAAG604.930109E-448.3333322
GTAACAA1109.8754754E-846.1363684
ACTGTCC951.9087329E-645.7894748
GCATACT951.9087329E-645.7894744
TAGATGT803.71776E-545.31254
TAAGGCT657.3144346E-444.6153837
Produced by FastQC (version 0.11.2)