FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_368.351000000a0f57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_368.351000000a0f57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118893
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT1540.12952823126676927No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC1330.11186529063948257No Hit
GTATATGCATACAATAGGATATTATTCATCCATAAAAGGGAATGAAGTAT1250.10513655135289714No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA404.778485E-9108.752
AGCACGC404.778485E-9108.751
CGCACTT451.0833901E-896.6666645
ACGCACT554.361027E-879.0909044
AACCGAA300.001931533972.499997
ACCGAAT300.001931533972.499998
CACGCAC607.9708116E-872.499993
TAACCGA300.001931533972.499996
TGCATAC604.9273967E-448.333336
TACAGAC803.7147E-545.31257
GTCTTGG657.310422E-444.6153831
TAACAAG1851.8189894E-1243.108115
AGTACAG855.3130236E-542.647065
GTAACAA2005.456968E-1239.8754
AGGATTC750.00147812738.6666688
TCATAAC1157.0897877E-637.8260843
TAGTAAC1803.1486707E-936.252
CAGACCC800.002029415536.259
TAATCTT850.002732320734.117654
GTATATG1501.122773E-633.8333361