FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_366.351000000a0f4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_366.351000000a0f4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110080
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT1510.13717296511627908No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCT100.0070823515145.02
AGAAGCG203.856821E-4108.753
CTATTAC300.001931152172.51
AAGCGGT350.00355807762.1428575
GAAGCGG400.00603664454.3754
TCATAAG400.00603664454.3752
TGTACTC400.00603664454.3753
GCTAACG400.00603664454.375145
CTATAGA604.925793E-448.3333321
GTTAAGG604.925793E-448.3333323
GAGTAAC700.001052543441.428571
TAGTAAC1406.538685E-736.252
GTAGTAA1759.953692E-833.1428571
CAGAAGC1102.4221587E-432.9545482
GTAACAA1601.8571191E-631.718754
TAGGATA1000.00605131129.04
TACAACA1000.00605131129.04
TAACAAG1855.7547277E-627.432435
CAGTGGG1100.0096349526.3636389
CTACATT1100.0096349526.3636384
Produced by FastQC (version 0.11.2)