FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_361.351000000a0f20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_361.351000000a0f20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123792
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG3370.2722308388264185No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2290.18498772133902028No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT2220.17933307483520744No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1870.1510598423161432No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1830.14782861574253586No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1610.13005686958769547No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1610.13005686958769547No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC1540.12440222308388263No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1500.1211709965102753No Hit
GTGCTTGTGATTCTAATTCACTTACATCATTGGCTCCCATTATGACCTCC1480.11955538322347163No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT1450.11713196329326613No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC1450.11713196329326613No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG1370.11066951014605145No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG1370.11066951014605145No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA1340.10824609021584594No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC1340.10824609021584594No Hit
ATACAAGGATATGACATGACCAAAGCTTGTTTCAAGGGAGACAGAGTAAA1330.1074382835724441No Hit
AGACAATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGGATGT1290.10420705699883677No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1280.10339925035543493No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT1280.10339925035543493No Hit
GTAATAAGTGACATGAATTTTCTTGATGAAGAAGGAAAAGCATATACAGC1250.10097583042522941No Hit
GCTTTATGGTCTGGCAGTTAAAGGACAATCTCATCTGAGAGGAGATACTG1250.10097583042522941No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG1240.10016802378182757No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT100.0070834095145.06
GAAACAC350.003559124462.1428574
AAACACG400.00603841454.3755
GCTGTCC1109.864743E-846.1363688
CAGAAGC657.311594E-444.6153872
CTGTCCC1151.3991848E-744.1304369
GGCTTGG1151.3991848E-744.1304361
CTATACA500.001392581343.59
CCTTATA700.001053052441.428572
AGTTCCT700.001053052441.428579
TTATTCA1303.663481E-739.0384649
GTATGAA750.001478363438.6666641
ACTATAC600.00469935236.258
GTAATAG800.002029738936.253
TAACAAG1803.1523086E-936.255
GTAGTAA2302.5465852E-1134.6739161
GTGTCCT1501.1232696E-633.8333321
TATAGAG1102.4238952E-432.9545485
TAGTAAC2257.7852746E-1032.2222252
GGCCAGC900.003616053432.222229
Produced by FastQC (version 0.11.2)