FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_348.351000000a0f13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_348.351000000a0f13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121974
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT1430.11723809992293441No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT1290.10576024398642334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT700.0124.2857065
GTAGTAA2200.092.2727361
AGCACGC950.091.578951
GCACGCA1000.087.02
AGCGCCA259.3668536E-487.05
ACGAGCG259.3668536E-487.04
TAACAAG2400.081.56255
GTAACAA2400.081.56254
CTGTCGT554.3621185E-879.090919
ACGCACT1100.079.090914
TAGTAAC2500.078.32
AAGCGGA300.001931654972.52
CGCTCTG702.7848728E-972.499995
TCGCTCT754.807589E-967.666674
CACGCAC1300.066.923073
AGTAACA3100.063.1451573
CTCGCTC851.2925739E-859.705883
GCACTTG1007.1122486E-1058.06
AACACGA1400.056.9642831
GTCTCGC902.0296284E-856.3888851
Produced by FastQC (version 0.11.2)